C14 Autoradiography; Glucose variable
This is the same model as C14 Autoradiography described above, except that a correction for the changing of plasma glucose during the experiment is included.
The autoradiographic experiment is performed as follows:
- The 14C-labeled deoxyglucose is injected.
- Blood is sampled and counted until the end of the experiment.
- The glucose concentration in plasma is measured for several samples.
- After 50 minutes the deoxyglucose has been trapped and the animal is sacrificed.
- The brain is isolated, then frozen, and sectioned into very thin slices.
- The slices are put onto a flat support and mounted into a radioactivity counter together with reference sheets of known activity concentration.
- The radioactivity is counted during several days.
The result is a set of images either on a conventional film or as a digital file in one of the popular graphic formats. These images can be turned into radioactivity units by a translation table which needs to be obtained from the reference sheets.