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Genarally, the one-way analysis of variance (Anova) is used to determine whether there are any significant differences between the means of three or more independent (unrelated) groups. This guide will provide a brief introduction to the one-way Anova, including the assumptions of the test and how you should prepare your data to use the implementation available in PMOD R. We will then show you how to run a one-way Anova for 3 groups in PMOD R interface using an appropriate example, which options to choose and how to interpret the output.